Suomeksi| PhD, Senior Scientist | |
| Veterinary Microbiology and Epidemiology, Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki | |
| probiotic lactobacilli, pilus and cell surface-exposed proteins, recombinant expression, functional and structural characterization |
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| 09-191 571 80 |
| Dr. Ingemar von Ossowski is a senior scientist in the Veterinary Microbiology and Epidemiology division at the Department of Veterinary Biosciences (University of Helsinki). He majored in microbiology and obtained his BSc (First Class Honors) (1984), MSc (1986), and PhD (1993) degrees from the Department of Microbiology at the University of Manitoba (CANADA) and has more than 18 years of research experience from multiple positions as a postdoctoral and research scientist. His overall research activities and experience are in the areas of molecular biology and protein chemistry, with a special interest in protein engineering and structural-related projects. He has worked at the molecular and biochemical level with several enzymes and proteins and become experienced with the use of a variety of recombinant protein expression hosts and the computer software and programs employed in sequence comparison, database searching, homology analyses, and visualizing 3D protein structures. He has also worked in both academic and industrial research laboratories, as well as internationally having had conducted research for extended periods in four different countries. His most recent scientific pursuit entailed an investigation into understanding the molecular mechanisms governing the intestinal mucosa adhesion interactions between a probiotic Lactobacillus spp. and its human host, a study that was primarily focused on the characterization of mucus-specific surface-exposed proteins. One of his current duties is the part-time administrative coordinator for the Center of Excellence in Microbial Food Safety research at the University of Helsinki. | |
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ARTICLES IN REFEREED SCIENTIFIC JOURNALS von Ossowski Ia, Reunanen J, Satokari R, Vesterlund S, Kankainen M, Huhtinen H, Tynkkynen S, Salminen S, de Vos WM, and Palva Aa (2010) Mucosal adhesion properties of the probiotic Lactobacillus rhamnosus GG SpaCBA and SpaFED pilin subunits. Appl. Environ. Microbiol. 76:2049-2057. Kankainen Mb, Paulin Lb, Tynkkynen S, von Ossowski Ib, Reunanen J, Partanen P, Satokari R, Vesterlund S, Hendrickx APA, Lebeer S, De Keersmaecker SJ, Vanderleyden J, Hämäläinen T, Laukkanen S, Salovuori N, Ritari J, Alatalo E, Korpela R, Mattila-Sandholm T, Lassig A, Hatakka K, Kinnunen KT, Karjalainen H, Saxelin M, Laakso K, Surakka A, Palva A, Salusjärvi T, Auvinen P, and de Vos WM (2009) Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili encoding a human-mucus binding protein. Proc. Natl. Acad. Sci. USA 106:17193-17198. von Ossowski I, Oksanen E, von Ossowski L, Cai C, Sundberg M, Goldman A, and Keinänen K (2006) Crystal structure of the second PDZ domain of SAP97 in complex with a GluR-A C-terminal peptide. FEBS J. 273:5219-5229. von Ossowski L, Tossavainen H, von Ossowski I, Cai C, Aitio O, Fredriksson K, Permi P, Annila A, and Keinänen K (2006) Peptide binding and NMR analysis of the interaction between SAP97 PDZ2 and GluR-A: Potential involvement of a disulfide bond. Biochemistry 45:5567-5575. von Ossowski Ib, Eaton JTb, Czjzek M, Perkins SJ, Frandsen TP, Schulein M, Panine P, Henrissat B, and Receveur-Brechot V (2005) Protein disorder: conformational distribution of the flexible linker in a chimeric double cellulase. Biophys. J. 88:2823-2832. von Ossowski I, Ståhlberg J, Koivula A, Piens K, Becker D, Boer H, Harle R, Harris M, Divne C, Mahdi S, Zhao Y, Driguez H, Claeyssens M, Sinnott M, and Teeri TT (2003) Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D. J. Mol. Biol. 333:817-829. Ducros VMA, Tarling CA, Zechel DL, Brzozowski AM, Frandsen TP, von Ossowski I, Schülein M, Withers SG, and Davies GJ (2003) Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7B E197A and E197S glycosynthase mutants. Chem. Biol. 10:619-628. Varrot A, Frandsen TP, von Ossowski I, Boyer V, Cottaz S, Driguez H, Schülein M, and Davies GJ (2003) Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens. Structure 11:855-864. von Ossowski I, Teeri T, Kalkkinen N, and Oker-Blom C (1997) Expression of a fungal cellobiohydrolase in insect cells. Biochem. Biophys. Res. Commun. 233:25-29. Loewen PC, Switala J, von Ossowski I, Hillar A, Christie A, Tattrie B, and Nicholls P (1993) Catalase HPII of Escherichia coli catalyzes the conversion of protoheme to cis-heme d. Biochemistry 32:10159-10164. von Ossowski I, Hausner G, and Loewen PC (1993) Molecular evolutionary analysis based on the amino acid sequence of catalase. J. Mol. Evol. 37:71-76. Loewen PC, von Ossowski I, Switala J, and Mulvey MR (1993) KatF (σs) synthesis in Escherichia coli is subject to posttranscriptional regulation. J. Bacteriol. 175:2150-2153. von Ossowski I, Mulvey MR, Leco PA, Borys A, and Loewen PC (1991) Nucleotide sequence of Escherichia coli katE, which encodes catalase HPII. J. Bacteriol. 173:514-520. a Corresponding author. ARTICLES IN CONFERENCE PROCEEDINGS Teeri TT, Divne C, Jones TA, Kleywegt G, Koivula A, Linder M, Ståhlberg J, von Ossowski I, Wohlfahrt G, and Zou J-Y (1999) Engineering the catalytic and binding properties of the cellobiohyrolases from Trichoderma reesei, p. 303-308. In Gilbert HJ, Davies GJ, Henrissat B, and Svensson B (eds.), Recent Advances in Carbohydrate Bioengineering. The Royal Society of Chemistry, Cambridge, UNITED KINGDOM. PATENTS de Vos, WM, Palva A, Palva I, Reunanen J, von Ossowski I, Satokari R, Vesterlund S, Kankainen M, Salusjärvi T, Tynkkynen S (2010) Novel peptides and methods for producing them. WO2010086512; US2010196524. Pedersen H, Lamsa M, Hansen PK, Frisner H, Vind J, Ernst S, Kongsbak L, Jørgensen BR, Beck TC, Husum TL, von Ossowski I (2001) Microtiter plate (MTP) based high throughput screening (HTS) assays. WO0132844; EP1230348; CA2388982; AU7646200. INVITED PRESENTATIONS von Ossowski I (2010) Comparative genomics brings to light a piliated probiotic Lactobacillus strain. Advanced PhD-training Course: Next Generation Genomics. (January 14-15th) University of Helsinki, Helsinki, FINLAND. von Ossowski I (2009) Characterization of Lactobacillus rhamnosus GG as a piliated probiotic isolate. The University of Helsinki Center of Excellence in Microbial Food Safety Research (MiFoSa) Seminar and Retreat. (August 25-26th) Lammi Biological Station, Lammi, FINLAND. von Ossowski I (2009) From secretome to interactome: molecular analysis of probiotic mechanisms of Lactobacillus rhamnosus GG (LGG). The ELVIRA (Nutrition, Food, and Health Research Programme) Annual Seminar on Nutrigenomics and Utilizing Postgenomic and Related Technologies in Nutrition and Food Studies. (March 19th) Sokos Hotel Vantaa, Vantaa, FINLAND. POSTER PRESENTATIONS Claes IJ, Lebeer S, Schoofs G, Depreeuw J, Rolain T, Hols P, von Ossowski I, Vanderleyden J, and De Keersmaecker SC (2010) Cell wall hydrolase activity of the major secreted proteins of the probiotic Lactobacillus rhamnosus GG. 3rd American Society for Microbiology Conference on Beneficial Microbes, James L. Knight Center and Miami Convention Center, Miami, Florida, UNITED STATES OF AMERICA. (non-attendee) Lebeer S, Verhoeven TL, Marien E, von Ossowski I, Reunanen J, Tynkkynen S, de Vos WM, De Keersmaecker SC and Vanderleyden J (2010) Reach out and touch down: the pili of the probiotic Lactobacillus rhamnosus GG are key adhesins that mediate immunomodulatory interactions with epithelial cells. 3rd American Society for Microbiology Conference on Beneficial Microbes, James L. Knight Center and Miami Convention Center, Miami, Florida, UNITED STATES OF AMERICA. (non-attendee) Reunanen J, von Ossowski I, de Vos WM, and Palva A (2010) Structure of mucus-binding pili in Lactobacillus rhamnosus GG. 13th International Society for Microbial Ecology Conference, Washington State Convention and Trade Center, Seattle, Washington, UNITED STATES OF AMERICA. (non-attendee) von Ossowski I, de Vos WM, Satokari R, Reunanen J, Kojo K, Vesterlund S, Huhtinen H, Kankainen M, Tynkkynen S, Salminen S, and Palva A (2008) From secretome to interactome: molecular analysis of probiotic mechanisms of Lactobacillus rhamnosus GG (LGG). The Ninth Symposium on Lactic Acid Bacteria, Hotel Zuiderduin, Egmond aan Zee, THE NETHERLANDS. von Ossowski I, Satokari R, Kojo K, Vesterlund S, Reunanen J, Huhtinen H, Salminen S, de Vos WM, and Palva A (2008) From secretome to interactome: molecular analysis of probiotic mechanisms of Lactobacillus rhamnosus GG (LGG) – An introduction and the present status. The ELVIRA (Nutrition, Food, and Health Research Programme) Annual Seminar, Hotel Presidentti Auditorium, Helsinki, FINLAND. Varrot A, Frandsen TP, von Ossowski I, Fort S, Boyer V, Fruchard S, Driguez H, and Davies GJ (2002) Thiooligosaccharides: probes for the study of glycoside hydrolases mechanism. 21st International Carbohydrate Symposium, Cairns Convention Centre, Cairns, AUSTRALIA. (non-attendee) Ståhlberg J, Koivula A, Wolhfahrt G, Boer H, von Ossowski I, Mäntylä A, Suominen P, Fagerström R, Jones TA, Harris M, Mahdi S, Divne C, Zou J-Y, Puls J, Sinnott M, Becker D, Claeyssens M, Piens K, Nerinckx W, Vasella A, Pipelier M, Vonhoff S, Lorthois E, and Teeri TT (2001) Engineering the catalytic properties of Trichoderma reesei cellobiohydrolases: Summary of an EU-project. 4th Carbohydrate Bioengineering Meeting, Royal Institute of Technology, Stockholm, SWEDEN. (non-attendee) Frandsen TP, Henrissat B, Ernst S, von Ossowski I, Andersen KV, and Schülein M (1999) Stopped-flow fluorescence investigation of cellulase: Substrate interactions using cellobiohydrolase Cel6A and endoglucanase Cel6B from Humicola insolens. 3rd Carbohydrate Bioengineering Meeting, University of Newcastle Upon Tyne, Newcastle, UNITED KINGDOM. von Ossowski I, Teeri,T, Kalkkinen N, and Oker-Blom C (1997) Expression of a fungal cellobiohydrolase in insect cells. 1st Annual Conference of the Swedish Structural Biology Network (SBNet), Tällberg, SWEDEN. von Ossowski I, Switala J, Hillar A, Tattrie B, Nicholls P, and Loewen P (1993) Probing the active site of E. coli Catalase HPII by site-directed mutagenesis. 36th Annual Meeting for the Canadian Federation of Biological Societies, Cleary International Centre, Windsor, Ontario, CANADA. (non-attendee) von Ossowski I, Sorby PA, Mulvey MR, and Loewen PC (1990) Characterization and sequencing of katE from E. coli. 40th Annual Canadian Society of Microbiology Meeting, University of Calgary, Calgary, Alberta, CANADA. von Ossowski I, Campbell C, Friesen AD, and Langstaff JM (1988) Purification and characterization of human Cu/Zn superoxide dismutase from yeast. 31st Annual Meeting for the Canadian Federation of Biological Societies, University of Laval, Quebec City, Quebec, CANADA. von Ossowski I, Campbell C, Friesen AD, and Langstaff JM (1987) Production of high levels of human Cu/Zn superoxide dismutase in yeast. International Symposium on Free Radicals in Health and Disease, St. Boniface General Hospital Research Centre, Winnipeg, Manitoba, CANADA. UPDATED: 6 Nov 2010 |
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